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Journal: Bioactive Materials

Article Title: Biodegradable Mg 2+ -releasing piezoelectric scaffold for segmental bone defect repair

doi: 10.1016/j.bioactmat.2026.02.017

Figure Lengend Snippet: Preparation and characterization of Mg 2+ -releasing piezoelectric scaffolds. A) Schematic illustration showing the preparation process for PWH Gel. B) XRD patterns and C) FTIR spectra of WH NP and PWH NP. D) Representative SEM image and elemental mapping of PWH NP, demonstrating uniform distribution of the characteristic Ca, P, O and Mg element. E) 1 H NMR spectra of gelatin and GelMA. F) Representative SEM images and elemental mapping of PWH Gel, showing highly interconnected porous architecture and homogeneous element dispersion. G) Micro-CT reconstruction illustrating the three-dimensional interconnected porous structure of PWH Gel. H-I) Voltage and current outputs of the WH Gel and PWH Gel under pressure. J-K) COMSOL finite element analysis simulation showing the stress and electric field distribution under external compression. L) Photograph showing the piezoelectric response effect demonstrated by lighting a bulb. M) In vivo piezoelectric testing in a rat radial defect under cyclical compression at 10 N.

Article Snippet: The collected radius samples were scanned and imaged using a micro-CT scanner (SkyScan1276, Bruker, China) and a digital X-ray machine (Parameter 3D, Kubtec, USA).

Techniques: Dispersion, Micro-CT, In Vivo

Journal: Bioactive Materials

Article Title: Biodegradable Mg 2+ -releasing piezoelectric scaffold for segmental bone defect repair

doi: 10.1016/j.bioactmat.2026.02.017

Figure Lengend Snippet: In vivo assessments of large segmental bone defect regeneration using Mg 2+ -releasing piezoelectric scaffold. A-B) Schematic showing the surgical procedure for scaffold implantation in rat radial defects (Scale bar = 1 cm). C) Macroscopic images of the defect site at 6- and 12- weeks post-implantation. D) RUS scores for radial repair. E) 3D micro-CT images of the defects at 6- and 12- weeks post-implantation (Scale bar = 3 mm). F-G) Quantitative micro-CT analysis of BV/TV and trabecular number (Tb.N) in cryogel-treated regions at 6- and 12- weeks post-implantation. H) Representative H&E and Masson's trichrome staining images of defect tissues at 6- and 12-weeks post-implantation (Scale bar: 1 mm). I) Immunohistochemical staining for COL-I (Scale bar: 1 mm). J) Representative immunofluorescence staining of CD31 (Scale bar: 1 mm). Data are expressed as mean ± S.D. (n = 3 independent replicates). Statistical significance was determined as ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001; NS, not significant.

Article Snippet: The collected radius samples were scanned and imaged using a micro-CT scanner (SkyScan1276, Bruker, China) and a digital X-ray machine (Parameter 3D, Kubtec, USA).

Techniques: In Vivo, Micro-CT, Staining, Immunohistochemical staining, Immunofluorescence